Figure 5
(A) Dynamic light scattering analysis of 120 μg/mL LDH in 0.2 M Tris–HCl buffer (pH 7.4) after the freeze–thaw process was repeated five times. The freezing rates were 0.2, 12.8, and 70.6 °C/min. (B) Nile Red fluorescence micrographs of 20 μg/mL LDH in 0.2 M Tris–HCl (pH 7.4) after the freeze–thaw process was repeated five times. LDH solution was frozen at a freezing rate of 0.2, 12.8, or 70.6ºC/min. The frozen samples were thawed in a water bath at 25 ºC for 5 min before analysis.
