Table 1 Showing the unbinding mechanical properties of HB-PNIPAM-Van-S. aureus, S. aureus and poly-DOPA functionalised probes during removal from the corneal surface using AFM.
Specimen/Stage | Energy dissipation (× 106 J) | Breaking force (nN) | No. of fitted segments (FS) | ΔL (nm) | Contour length (μm) | FSSR (%) | FSLR (%) | Xmax > 2.5 μm (%) |
|---|---|---|---|---|---|---|---|---|
HB-PNIPAM-Van-S. aureus Stage 1 | 212 ± 174 | 0.5 ± 0.4 | 1206 | 109 ± 105 | 1.9 ± 1.3 | 69 | 31 | 10.78 |
HB-PNIPAM-Van-S. aureus Stage 2 | 174 ± 154* | 0.8 ± 0.6* | 2601 | 75 ± 68* | 2.1 ± 1.2* | 64 | 36 | 9.02 |
HB-PNIPAM-Van-S. aureus Stage 3 | 232 ± 151* | 0.7 ± 0.5* | 2731 | 73 ± 69 | 1.1 ± 0.8* | 92 | 8 | 7.72 |
S. aureus Stage 1 | 155 ± 89 | 0.7 ± 0.6 | 3194 | 35 ± 30 | 1.6 ± 1.1 | 82 | 18 | 1.37 |
S. aureus Stage 2 | 167 ± 167 | 0.8 ± 0.7 | 3482 | 36 ± 31 | 1.6 ± 1.1 | 80 | 20 | 0.06 |
S. aureus Stage 3 | 184 ± 150* | 0.9 ± 0.7 | 4776 | 26 ± 18 | 1.3 ± 0.9 | 89 | 11 | 0 |
Poly-DOPA Stage 1 | 5 ± 1 | – | – | – | – | – | – | – |
Poly-DOPA Stage 2 | 5 ± 2 | – | – | – | – | – | – | – |
Poly-DOPA Stage 3 | 5 ± 2 | – | – | – | – | – | – | – |
