Table 1 HPLC Conditioned for Folic Acid and Vitamin B12 Analysis.
Column | Kinetex, 2.6 µm, C18, 100 Å, LC column 100 × 4.6 mm | |||
Dimensions | 100 × 4.6 mm ID | |||
Elution type | Gradient | |||
Acetonitrile | ||||
Elution B: | 20 mM phosphate buffer pH 3 | |||
Gradient profile | Step No | Time (min) | Sol. A | Sol. B |
0 | 0.5 | 5 | 95 | |
1 | 5.0 | 25 | 75 | |
2 | 5.00 | 5 | 95 | |
3 | 5.00 | 75 | 25 | |
4 | 5.00 | 5 | 95 | |
Flow rate | 1 mL/min | |||
Col. Temp | Ambient | |||
Detection | UV–Vis Abs.-variable wave.(UV) @ 278 & 360 nm (22 °C) | |||
Run time | 20 min | |||
Injection volume | 20µL | |||
