Table 1 Primers and conditions of PCR.

From: High prevalence of blaCTX-M and blaSHV among ESBL producing E. coli isolates from beef cattle in China’s Sichuan-Chongqing Circle

Primer

PCR primers (5′ → 3′)

Expected size (bp)

PCR conditions

Ref.

CTX-M-F

ACGCTGTTGTTAGGAAGTG

759 bp

94 °C, 5 min; 35 cycles of 94 °C, 45 s, 58 °C, 45 s, 72 °C, 1 min

36

CTX-M-R

TTGAGGCTGGGTGAAGT

SHV-F

TCGGGCCGCGTAGGCATGAT

626 bp

94 °C, 10 min; 35 cycles of 94 °C, 1 min, 60 °C, 1 min, 72 °C, 1 min

37

SHV-R

AGCAGGGCGACAATCCCGCG

TEM-F

ATAAAATTCTTGAAGACGAAA

1080 bp

94 °C, 5 min; 35 cycles of 94 °C, 1 min, 58 °C, 1 min, 72 °C, 1 min

38

TEM-R

GACAGTTACCAATGCTTAATCA

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