Figure 1
From: Local application of Usag-1 siRNA can promote tooth regeneration in Runx2-deficient mice
Silencing with Usag-1 and Runx2 Stealth siRNAs and the effects on tooth formation. (A) Usag-1 expression in mHAT9d cells at 48 h post-transfection of Usag-1 Stealth siRNAs (#903 and #304), as determined by sqRT-PCR. Both siRNAs showed ~ 50% knockdown efficiency. The synthesized complementary DNA (cDNA) samples were serially diluted and subjected to semi-quantitative PCR analysis. 1/10, 1/30, and 1/90 indicates dilution ratio. (B) Usag-1 expression in organ cultures treated with Usag-1 Stealth siRNAs (#903 and #304), as determined by sqRT-PCR. The knockdown efficiencies of both siRNAs in E10 mandible explant cultures were comparable to those in mHAT9d cells. (C) The most advanced developmental stage and number of tooth germs in organ cultures 10 days after co-administration of Usag-1 and Runx2 Stealth siRNAs. Developmental stages were classified as no tooth formation, the bud stage, or the cup stage.
