Figure 5
From: Local application of Usag-1 siRNA can promote tooth regeneration in Runx2-deficient mice
Immunohistochemical evaluation of serial sections and sqRT-PCR analysis from renal capsules transplanted with mandibles from E10 Runx2-KO mice and treated with Stealth siRNAs. (A-a, b) H&E staining showed no tooth structures; however, odontogenic epithelial-like cells with elongated, rectangular morphology were observed. Magnification: 200× and 400× . (A-c) Immunostaining for amelogenin was positive in odontogenic epithelial-like cells. Magnification: 400× . (A-d) Control. (B) sqRT-PCR analysis of the enamel-specific proteins, amelogenin and ameloblastin. sqRT-PCR at 19 days post-transplantation, using wild-type and Runx2-KO mouse mandibles with Usag-1 Stealth siRNA #304 or PBS.
