Figure 6

PRR is translocated to the cell plasma membrane in collecting ducts of STZ rats. (A) Representative image of PRR immunohistochemistry in medullary kidney slides of control and STZ diabetic rats. As observed, PRR is mostly observed on the apical side of the collecting duct cells (arrows) in STZ rats, while in control rats PRR is present more diffusely in the cytoplasm. (B) Representative immunoblots (n = 3) showing the increase of PRR abundance in the plasma membrane fractions in medullary tissues of STZ rats. E-cadherin was used as a constitutive protein for plasma membrane fractions. *P < 0.05 versus control rats.