Figure 1 | Scientific Reports

Figure 1

From: Zfp57 inactivation illustrates the role of ICR methylation in imprinted gene expression during neural differentiation of mouse ESCs

Figure 1

Differentiation protocol and marker expression analysis of wild-type and Zfp57-/- hybrid cells. (a) Schematic of neural differentiation. Wild-type and Zfp57-/- JB1 ESCs were cultured in DDM medium for 12 days and 1 μM cyclopamine was added from day 2 to day 10 to induce dorsalization of progenitor cells. At day 12 neural progenitor cells were harvested. (b–d) Expression analysis of pluripotency (b), neural (c) and endoderm-mesoderm (c) differentiation markers and imprinted genes (e,f) in wild-type and Zfp57-/- JB1 ESCs (day 0) and NPCs (day 12) assayed by quantitative RT-qPCR. The histograms show the average gene expression levels of three independent experiments, after normalization against the level of β-actin. Error bars represent the SD. Note that Zfp57 expression was detected with the use of primers external to the deletion introduced during the gene knockout.

Back to article page