Table 2 HTL epitopes predicted using NetMHC II pan 3.2 server. Position indicates the starting residue of the proteins being studied. Epitopes with score ≤ 2.0 are considered as strong binders and epitopes with antigenicity score > 0.4 are considered to be antigenic which have the possibility of generating an effective immune response.
From: In silico designing of vaccine candidate against Clostridium difficile
HTL EPITOPES | |||||
|---|---|---|---|---|---|
Protein | Epitopes | Position | Allele | Score | Antigenic score |
FliC | MRVNTNVSALIANNQ | 1 | DRB1*13:02 | 0.50 | 0.4654 |
DRB3*02:02 | 0.90 | ||||
DQA1*01:02-DQB1*06:02 | 0.60 | ||||
NNNEIKIQLVNTASI | 161 | DRB1*04:01 | 2.00 | 0.9979 | |
DRB1*08:02 | 1.90 | ||||
DRB1*13:02 | 1.50 | ||||
DRB4*01:01 | 0.20 | ||||
NNEIKIQLVNTASIM | 162 | DRB1*04:01 | 1.10 | 0.7263 | |
DRB1*04:05 | 1.90 | ||||
DRB1*08:02 | 0.90 | ||||
DRB1*12:01 | 1.80 | ||||
DRB1*13:02 | 0.50 | ||||
DRB4*01:01 | 0.10 | ||||
CotE | NNQILRTILYYPLTT | 133 | DRB1*12:01 | 1.00 | 0.4123 |
DPA1*02:01-DPB1*01:01 | 1.90 | ||||
DPA1*01:03-DPB1*02:01 | 1.80 | ||||
DPA1*01:03-DPB1*04:01 | 1.60 | ||||
DPA1*03:01-DPB1*04:02 | 1.80 | ||||
AQLLDAVIFAFAEID | 396 | DQA1*05:01-DQB1*02:01 | 1.00 | 0.4179 | |
DQA1*03:01-DQB1*03:02 | 0.40 | ||||
DQA1*04:01-DQB1*04:02 | 0.40 | ||||
KDGDFAMSYDDALSI | 645 | DRB3*01:01 | 1.30 | 0.5761 | |
DQA1*03:01-DQB1*03:02 | 1.70 | ||||
DQA1*01:01-DQB1*05:01 | 0.40 | ||||
DQA1*05:01-DQB1*02:01 | 0.10 | ||||
SlpA | RYETSLAIADEIGLD | 513 | DQA1*05:01-DQB1*02:01 | 0.25 | 0.7434 |
DQA1*03:01-DQB1*03:02 | 0.07 | ||||
DQA1*04:01-DQB1*04:02 | 0.07 | ||||
ETSLAIADEIGLDND | 515 | DQA1*05:01-DQB1*02:01 | 1.30 | 0.6268 | |
DQA1*03:01-DQB1*03:02 | 1.00 | ||||
DQA1*04:01-DQB1*04:02 | 1.40 | ||||
IAGRFKESPAPIILA | 660 | DRB1*07:01 | 0.60 | 0.4282 | |
DRB1*09:01 | 0.50 | ||||
DRB3*02:02 | 1.80 | ||||
DPA1*02:01-DPB1*14:01 | 1.40 | ||||
