Figure 1

Twofold titration of monoclonal antibody (CCHF_mAb1 and CCHF_mAb2) against recombinant NP in triplicates. Monoclonal antibody 1 (CCHF_mAb1) showed reactivity titre up to 1:1600 dilution against r-NP in indirect ELISA. Monoclonal antibody 2 (CCHF_mAb2) showed the reactivity up to 1:3200 dilution against r-NP in indirect ELISA with the cut-off value of 0.5 determined by twice the OD of negative control.