Figure 6 | Scientific Reports

Figure 6

From: Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray

Figure 6

Detection of DNA double strand breaks in tumor spheroids. (a) An assay to detect double strand DNA breaks. (b) OVCAR8 tumor spheroids incubated with IPO or nanoparticles without iodine were irradiated with 33.0, 33.2 and 33.4 keV monochromatic X-rays for 30 min. After irradiation, spheroids were fixed immediately and γH2AX foci assay was performed. Images of γH2AX foci reflecting DNA double strand breaks are displayed by blue (color) as well as by white (grayscale). Tumor spheroids incubated with nanoparticles that do not contain iodine (no iodine NP) were used as no iodine controls. Red fluorescence shows nanoparticles (NPs) labeled with rhodamine-B. (c) Quantitation of double strand DNA breaks plotted against the energy of X-ray. Mean ± s.e.m. (n = 3). FL fluorescence. (d) Detection of double strand DNA breaks using X-rays with 0.1 keV interval. OVCAR8 tumor spheroids incubated with IPO were irradiated with monochromatic X-rays for 30 min. After irradiation, spheroids were fixed immediately and γH2AX foci assay was performed. Mean ± s.e.m. (n = 3). FL fluorescence.

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