Figure 5
Expression regulation and anti-senescence analyses of MYB TFs. (a) qRT-PCR validation of MYB TFs in the YN0 versus YN30 comparison. (b) qRT-PCR validation of MYB TFs in the YB0 versus YB30 comparison. (c) qRT-PCR results of endogenous CHS and CHI genes in protoplasts transfected with OsR498G0101613100. Statistical analysis was performed using Student’s t-test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; n.s. not significant. (d) qRT-PCR validation of OsR498G0101613100, OsCHS and OsCHI between YN and YB. (e) Promoter activity analysis shows that OsR498G0101613100 significantly suppresses the transcriptional activity of CHS and CHI. ***, P < 0.001*, t-test. (f) CRISPR-Cas9-mediated knockout of OsR498G0101613100. The green box represents the exon. Sequences of target in the CRISPR-Cas9 transgenic lines of rice variety Zhonghua11 (ZH11) were shown. Wild type refers to the sequence of ZH11. #9, #15 refer to the different types of knockout mutants. (g) Leaves phenotype after darkness treatment on the ko9 and ko15 mutants and the wild-type control ZH11. Photographs were taken 5 days after darkness treatment. Bar, 2 cm.
