Figure 5
From: Attenuated asthma phenotype in mice with a fetal-like antigen receptor repertoire
Test protocol. Mice were sensitized to ovalbumin (OVA) by intraperitoneal injections on days 1, 14, and 21. To induce an allergic airway inflammation, mice received allergen challenges via the airways delivered by nebulization with OVA on days 26, 27 and 28. The control group received PBS instead of OVA. To quantify the immunoglobulin classes in serum using ELISA, blood samples were taken on the day before the first sensitization and on day 30. Head-out body plethysmography including methacholine provocation was used to assess the lung function on day 29. Bronchoalveolar lavage was performed on day 30. BAL fluids were evaluated for cytokine levels as well as eosinophil content.
