Table 5 Primary and secondary antibodies used for kidney immunohistochemistry.
Primary antibodies | Dilution and source | Secondary antibodies | Antibody target/marker |
|---|---|---|---|
Goat anti rat calcitonin gene-related peptide (CGRP) antibody | 1:500, Bio-Rad/AbD Serotec Cat# 1720–9007, RRID: AB_2290729 | Jackson ImmunoResearch Labs Cat# 715–165-151, RRID: AB_2315777 | CGRP |
Monoclonal mouse anti-Tyrosine Hydroxylase (TH) antibody | 1:200, Avanti Antibodies Cat# AV1, RRID: AB_2531895 | Jackson ImmunoResearch Labs Cat# 705–165-147, RRID: AB_2307351 | TH |
Polyclonal goat anti-renin antibody, IgG isotype | 1:500, Santa Cruz, Cat# sczsc27318, RRID: AB_2301005 | Jackson ImmunoResearch Labs Cat# 705–165-147, RRID: AB_2307351 or Jackson ImmunoResearch Labs Cat# 705–486-147, RRID: AB_2616594 | Renin |
Polyclonal rabbit anti-aquaporin 1 (AQP1) | 1:100, Alomone lab, Cat# AQP-001. RRID: AB_2039726 | Jackson ImmunoResearch Labs Cat# 711–166-152, RRID: AB_2313568 | Proximal convoluted tubule and thin descending limb of Henle’s loop |
Polyclonal rabbit anti-AQP2 | 1:2000, Alomone lab, Cat# AQP-002. RRID: AB_2039728 | Jackson ImmunoResearch Labs Cat# 711–166-152, RRID: AB_2313568 | Cortical and medullary collecting ducts |
Fluorescein-labelled peanut Agglutinin (PNA) | 1:2000, Vector Laboratories Cat# FL-1071, RRID: AB_2315097 | NA | Distal convoluted tubule |
