Figure 1

The expression level of miR-378a was elevated in active psoriasis. (a) The expression level of miR-378s in psoriatic lesions from Thai patients (n = 10) compared with healthy individuals (n = 10) was analyzed using an unpaired t-test. (b) The expression level of miR-378a in paired psoriatic skin lesions before and after treatment with MTX (blue; n = 7) and paired psoriatic skin lesions before and after treatment with NB-UVB (green; n = 3) were analyzed using a paired t-test. Expression levels of micro RNA were normalized with let-7a expression from the skin biopsies. The expression level of miR-378a in (c) immortalized human keratinocytes HaCaT cells, and (d) normal human keratinocytes (NHEK) both stimulated with synergistic cytokines (TNF-α + IL-17A) for 6, 12, 24, and 48 h. Expression levels of miRNA were normalized with RNU44 expression from HaCaT cells. Error bars indicate mean ± SEM, ***indicates P < 0.001, **indicates P < 0.01, and *indicates P < 0.05.