Figure 2
miR-378a diminished cell proliferation in NHEK cells, leading to increased apoptosis and cell cycle arrest. (a) The proliferation analysis of NHEK cells transiently expressing miR-378a for 24, 48, 72, and 96 h as measured by MTS assay. (b) The percentage of apoptosis keratinocytes after transfection with miR-378a for 72 h. The bar chart shows an average percentage of apoptotic cells calculated from three independent flow cytometry-based apoptosis assays. The dot plots illustrate the necrotic cells (Q1), late apoptotic cells (Q2), early apoptotic cells (Q3), and live cells (Q4). (c) The percentage of NHEK cells over-expressing miR-378a for 72 h at different cell cycle phases (G1, S, and G2). The bar chart shows an average of the percentage of cells calculated from three independent cell cycle assays as measured by flow cytometer. The histograms display a representation of cell cycle phase distribution in cells over-expressing miR-378a or negative control mimic. Error bars indicate mean ± SEM from three replicates, ****indicates P < 0.0001, ***indicates P < 0.001, and ns indicates non-significant. Neg ctrl mimic = negative control of miR-378a.
