Figure 3
From: Endothelial cell-derived Apelin inhibits tumor growth by altering immune cell localization
Tumor vessel formation and functional analysis. (A) Immunofluorescence staining of CD31 (green) in frozen sections of MC38 tumors from WT or Apelin-KO mice. Scale bar = 200 µm. (B) Quantification of total vessel length (6 random fields of 5 independent tumor sections). [edge; 158.05 ± 35.47 (WT) vs 122.14 ± 23.17 (KO), p = 0.04, center; 141.99 ± 21.99 (WT) vs 93.80 ± 13.07 (KO), p = 0.0003]. (C) Quantification of average vessel diameter of tumor central area (6 random fields of 5 independent tumor sections). (D) Immunofluorescence staining of CD31 (green) in frozen sections of MC38 tumors from WT mice receiving [Pyr1]Apelin-13 or saline infusions using osmotic pumps. Scale bar = 200 µm. (E) Quantification of total vessel length (6 random fields of 5 independent tumor sections). [edge; 152.10 ± 10.07 (Saline) vs 110.55 ± 21.27 ([Pyr1]Apelin-13), p = 0.0005, center; 118.93 ± 14.30 (Saline) vs 95.89 ± 21.04 ([Pyr1]Apelin-13), p = 0.03]. (F) Quantification of average vessel diameter of tumor central area (6 random fields of 5 independent tumor sections). (G) Immunofluorescence staining of CD31 (green) in MC38 tumor sections from WT and Apelin-KO mice. Hypoxic status was revealed by Hypoxyprobe-1 (red). Scale bar = 200 µm. (H) Quantification of the hypoxic area in MC38 tumor sections. Data are mean ± SD and were analyzed by two-sided Student's t-test. *p < 0.05, **p < 0.01.
