Figure 5

Global transcript expression levels of tumor endothelial cells analyzed by RNA sequencing. (A) Volcano plot of RNA-seq transcriptome data depicting gene expression patterns for ECs isolated from WT relative to Apelin-KO mice tumors at day 19 (n = 3 for each group). Volcano plot was illustrated by R 4.0.4 with packages ggplot2 and ggrepel. (B) Heatmap of 30 highly changed genes in ECs from WT or Apelin-KO mice bearing tumors (n = 3 for each group). Heatmap was drawn with log2 FPKM values. (C) qRT-PCR showing CCL8 expression levels in ECs and whole tumor tissue normalized to WT mouse tumor CCL8 expression. (D) qRT-PCR showing CCL8 expression levels in MC38 cancer cells, CD45⁺ CD31⁻ immune cells and CD45⁻ CD31⁻ stromal cells, respectively, normalized to WT mouse tumor CCL8 expression. (E) qRT-PCR of CCR expression levels in CD8⁺ and CD4⁺ T cells. The error bars indicate mean ± SD and all data were analyzed by two-sided Student's t-test. **p < 0.01.