Figure 3 | Scientific Reports

Figure 3

From: Similarities and differences between IL11 and IL11RA1 knockout mice for lung fibro-inflammation, fertility and craniosynostosis

Figure 3

Reduced activation of primary lung fibroblasts from Il11 knockout mice. Automated immunofluorescence quantification of (A) ACTA2+ve cells, (B) COL1A1 expression (intensity/area) and (C) EdU+ve cells in TGFβ1- or IL11-treated lung fibroblasts from Il11−/− or wild-type mice (5 ng/ml, 24 h). One representative dataset from two independent biological experiments is shown (14 measurements per condition per experiment). Scale bars: 200 µm. (D) Secreted collagen concentrations in supernatant of cells treated as described in (A,B) were quantified (n = 5). (E) Secreted IL11 in culture supernatant from TGFβ1-treated lung fibroblasts from Il11−/− or wild-type mice (5 ng/ml, 24 h; n = 3). Data in (A–D) are shown as: centre line, median value; box edges, 25th and 75th percentiles; whiskers, minimum and maximum values; and shown as mean ± SD in (E). P values in (A–D) were determined by ANOVA (Sidak’s test) and by ANOVA (Tukey’s test) in (E).

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