Figure 3
From: Mechanical activities of self-beating cardiomyocyte aggregates under mechanical compression
The dynamic properties of single cardiac spheroids (CSs). (a) The normalized beating frequency, ω/ω0, is plotted against the normalized indentation depth (i.e., effective compression strain) 2δz/D, where ω and ω0 are the beating frequencies under compression and no compression, respectively. The black broken line is a fitting function of ω/ω0 = 1 + a (2δz/D)n, where the fitting parameters were determined to be a = 4.7 and n = 2.8 by least square analysis. (b) The mechanical work performed by a single CS in a single contraction, termed beating energy Wbeat, is plotted against Dδz. The black broken line is a fitting function of Wbeat = b (Dδz)1.5, where the fitting parameter was determined to be b = 1.7 × 10–6 nJ/μm3 by least square analysis. The plotted data in (a) and (b) are from 94 contractions of six different CSs, the diameters of which are ranged from 219 to 355 μm.
