Figure 1
From: Immunomodulatory role of Parkinson’s disease 7 in inflammatory bowel disease
Presence of PARK7 in the colonic mucosa of children. The mRNA expression (a), protein level (b, g) and localization (c–f) of PARK7 were determined by real time PCR (a) (n = 10–13), Western blot (b) (n = 6–10) or immunohistochemistry (c–f, green) in the inflamed (iCD) and non-inflamed (niCD) colonic mucosa of children with Crohn’s disease (a, b, d, e), ulcerative colitis (UC) (a, b, f, g) and controls (a–c, g). Relative expression levels are presented in comparison with RPLP0 mRNA (a) or GAPDH protein (b, g) as internal control. The data are normalized against corresponding controls. Results are illustrated as mean + SEM, dots represent individual values. Analysis of significance was performed by ordinary one-way ANOVA test. #p < 0.05 comparing the connected groups. Full-length blots are included in “Supplementary Informations”. Cell nuclei were counterstained with Hoechst 33342 (blue). Scale bar: 60 µm or 20 µm. Arrowheads indicate crypt epithelial cells, * symbols indicate lamina propria.
