Figure 1
From: HIV envelope antigen valency on peptide nanofibers modulates antibody magnitude and binding breadth
Nanostructure and antigenicity of nanofiber-gp120 conjugates. (A) Schematic representation of gp120 antigens (maroon) covalently linked to fibrillizing peptides (blue). (B) TEM image of gp120 nanofibers stained with uranyl acetate. (C) Binding of antibodies against VRC01, B12, CH58, and CH22 epitopes to gp120 nanofibers prepared with different molar ratios of crosslinking agent SMCC, measured by ELISA. (D) Representation of gp120 antigenicity and loading density on nanofibers with selected formulation (10×) boxed in red. Antigenicity index for each condition was calculated by adding the AUC of ELISA binding curves from VRC01, B12, CH58 and CH22, then dividing by the sum AUC of unmodified gp120. (E) Dissociation rate constants (koff) of antibodies to gp120 and gp120 nanofibers measured by Biolayer Interferometry.
