Figure 2
From: HIV envelope antigen valency on peptide nanofibers modulates antibody magnitude and binding breadth
Nanofiber conjugation increases antibody binding breadth. (A) Magnitude of serum antibody responses to gp120 from mice immunized with gp120 or gp120 nanofibers at times indicated by arrows as measured by ELISA. Bold line shows mean titer and thin lines show individual responses. Mice received 4 total immunizations of 50 μg gp120 each. Responses were compared using a linear mixed effects model and no significant difference was observed. (B) Binding of serum antibodies to a panel of heterologous antigens at 6, 10, and 14 weeks post immunization. Groups were compared using GEE (see methods for more detail). (C) Avidity index of serum antibodies to gp120 measured by antibody binding under denaturing conditions. (D) Time progression of heterologous antigen binding displayed by summing the responses shown in (B). Groups were compared using a repeated measures non-parametric test with differences between treatment groups denoted on graph legends. *p < 0.05, **p < 0.01, ****p < 0.0001. (n = 4 animals/group for gp120-Q11 and gp120/Q11, n = 5 animals/group for gp120).
