Figure 4

High gp120 antigen density on nanofibers drives increased antibody titer and binding breadth. (A) Schematic representation of gp120 nanofibers and unassembled antigens modified with non-assembling peptides. (B) Number of gp120 antigens on gp120^high-Q11 and gp120^low-Q11 for 3 immunizations estimated by gp120 concentration and average fiber length. (C) Magnitude of serum antibody responses to gp120 from mice immunized with indicated formulations at times indicated by arrows as measured by ELISA. Bold line shows mean titer and thin lines show individual responses. Compared using a linear mixed effects model, *p < 0.05,**p < 0.01. (D) Binding of serum antibodies to a panel of heterologous antigens at 6-weeks post primary immunization. (E) Binding of serum antibodies to heterologous antigens from 19-weeks post primary immunization as in D. Groups were compared using GEE (see methods for more detail). **p < 0.01.