Figure 2
From: Mitofusion is required for MOTS‐c induced GLUT4 translocation
Treatment of U-2 OS cells with MOTS-c increased protein expression of (A) TFAM (B) NRF1 (C) COX4 but had no significant impact on (D) PGC1α and (E) TOMM20. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. GAPDH was used as the internal control. All full-length blots are presented in Supplementary Figure WB1. Data are means ± SEM of three experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, **p < 0.01 and ****p < 0.0001 vs. vehicle.
