Figure 3
From: Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans

Isoflurane increased ROS levels in wild-type C. elegans. (a) Isoflurane increased the levels of ROS in C. elegans as compared to the control condition. (b) Superoxide staining by MitoSOX shows an elevated level of superoxide of C. elegans following the treatment with isoflurane and control conditions. (c) The quantification of the staining showed that the isoflurane treatment (green bar) increased the level of superoxide compared to the control condition (blue bar) in C. elegans. All data are presented as mean ± S.D. N = 10 hermaphrodites in each group. The Student's t-test was used to analyze the data presented in (a,c); the P values refer to the difference between the control condition and isoflurane. * P < 0.05.