Figure 4
From: Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans
Isoflurane impaired oogenesis via an abl-1-associated oxidative stress-related pathway. (a) Treatment with 7% isoflurane for 4 h did not decrease the number of embryos produced by abl-1 knockout hermaphrodites. (b) Treatment with 7% isoflurane for 4 h did not decrease the number of unfertilized eggs produced by abl-1 knockout hermaphrodites. (c) Treatment with 5 mM N-acetyl-cysteine (NAC) in wild-type C. elegans mitigated the isoflurane-induced reduction in the number of embryos produced by N2 hermaphrodites. (d) Treatment with 5 mM NAC attenuated the isoflurane-induced reduction in the number of unfertilized eggs produced by N2 hermaphrodites. All data are presented as mean ± S.D. N = 10 hermaphrodites in each group. The Student's t-test was used to analyze the data presented in (a,b); the P values refer to the difference between the control condition and isoflurane. Two-way ANOVA and posthoc Bonferroni test was used to analyze the data presented in (c,d); the P values refer to the interaction of group (control condition versus isoflurane) and treatment (vehicle versus NAC), and the difference between each treatment. ** P < 0.01, *** P < 0.001. XR1: C. elegans strain abl-1(ok171); NAC N-acetyl-L-cysteine.
