Figure 5
From: Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans
Mating with male C. elegans did not attenuate the isoflurane-induced changes in oogenesis. (a) Wild-type N2 hermaphrodites were treated with 7% isoflurane for 4 h. Then, five male C. elegans were transferred into each plate for mating with one hermaphrodite for 12 h. The number of embryos was significantly lower in the isoflurane group than the control group with or without mating with male C. elegans. (b) Five male C. elegans per plate were treated with 7% isoflurane, and then every five male C. elegans mated with one hermaphrodite for 12 h. There was no significant difference in the number of embryos between the control and isoflurane groups. All data are presented as mean ± S.D., N = 10 hermaphrodites in each group. Two-way ANOVA and posthoc Bonferroni test was used to analyze the data shown in (a); the P values refer to the interaction of group (control condition versus isoflurane) and treatment (with versus without male C. elegans). The Student's t-test was used to analyze the data presented in (b); the P values refer to the difference between the control condition and isoflurane.
