Table 1 Reproducibility assay of the HRM method.

From: Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis

No.

Intra-assay reproducibility

Inter-assay reproducibility

Tm1 mean

\(\overline{\upchi }\) ± SD

CV/%

Tm2 mean \(\overline{\upchi }\) ± SD

CV/%

Tm1 mean

\(\overline{\upchi }\) ± SD

CV/%

Tm2 mean

\(\overline{\upchi }\) ± SD

CV/%

1 × 10–2

69.53 ± 0.06B

0.08

71.04 ± 0.09A

0.14

69.47 ± 0.06B

0.08

71.08 ± 0.08A

0.11

1 × 10–3

69.46 ± 0.06B

0.08

70.95 ± 0.09A

0.12

69.48 ± 0.08B

0.11

70.98 ± 0.08A

0.11

1 × 10–4

69.41 ± 0.08B

0.11

70.93 ± 0.06A

0.08

69.53 ± 0.06B

0.08

71.01 ± 0.1A

0.14

  1. Different uppercase letters in the same row mean extremely significant difference (P = 0.000 < 0.01).
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