Table 1 NOEC (no observed effect concentration) and LOEC (lowest observed effect concentration) levels (mol/L) for development stages (fertilized egg, trochophore, 48 and 72 h-old D-larva) of Venerupis corrugata, Ruditapes decussatus and Ruditapes philippinarum after exposure to increasing concentrations of the following cryoprotecting agents (CPAs): ethylene–glycol (EG), propylene–glycol (PG), dymethil-sulfoxide (Me2SO) and glycerol (GLY).
From: Larval cryopreservation as new management tool for threatened clam fisheries
Fertilized egg | Trochophore | 48 h-old D-larva | 72 h-old D-larva | |||||
---|---|---|---|---|---|---|---|---|
NOEC (M) | LOEC (M) | NOEC (M) | LOEC (M) | NOEC (M) | LOEC (M) | NOEC (M) | LOEC (M) | |
Venerupis corrugata | ||||||||
Me2SO | – | 0.5 | 2 | 3 | 2 | 3 | 2 | 3 |
GLI | – | 0.5 | 1.5 | 2 | 2 | 3 | 2 | 3 |
EG | 3 | – | 3 | – | 3 | – | 2 | 3 |
PG | – | 0.5 | 3 | – | 3 | – | 3 | – |
Ruditapes decussatus | ||||||||
Me2SO | – | 0.5 | 2 | 3 | 2 | 3 | 2 | 3 |
GLI | – | 0.5 | 1 | 1.5 | 1.5 | 2 | 2 | 3 |
EG | 0.5 | 1 | 3 | – | 3 | – | 2 | 3 |
PG | - | 0.5 | 2 | 3 | 3 | – | 3 | – |
Ruditapes philipinarum | ||||||||
Me2SO | – | 0.5 | 1.5 | 2 | 2 | 3 | 2 | 3 |
GLI | – | 0.5 | 0.5 | 1 | 2 | 3 | 3 | – |
EG | – | 0.5 | 2 | 3 | 3 | – | 3 | – |
PG | 0.5 | 1 | 1.5 | 2 | 3 | – | 3 | – |