Figure 2 | Scientific Reports

Figure 2

From: Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells

Figure 2

Preparation of heat-inactivated F. nucleatum and attachment of F. nucleatum on the surface of OSCC cells. (A) Pictures of F. nucleatum colonies. The heat-inactivated F. nucleatum bacterial cells were prepared by heating for 15 min at 100 °C. Bacterial cells show no colony forming on sheep blood agar after heat inactivation. (B) Attachment of F. nucleatum on the surface of OSCC cells. Green fluorescent-labeled live or heat-inactivated F. nucleatum (JCM8532 and ATCC10953) were co-culture with OSCC cells for 3 h. After washing by PBS, attached cells on the surface of OSCC cells were checked by immunofluorescence microscopy. Images show that heat-inactivated or live bacterial cells attach on the surface of OSCC cells. Moreover, invaded bacterial cells into OSCC cells were examined by culture on blood agar after treatment with antibiotics. Table shows the number of invaded F. nucleatum (CFU/ml) in OSCC cells (HOC621, HSC2, HOC719-PE, and HOC719-NE).

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