Figure 7 | Scientific Reports

Figure 7

From: VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, induces cell cycle arrest and apoptosis in breast cancer cells by inhibiting the Wnt/β-catenin pathway

Figure 7

Effects of VALD-3 on the anti-tumor in vivo. (A) Tumor formation in nude mice. (B) Body weight were measured every 3 days. (C) Tumor volume variation, **p < 0.05 versus control group. Each point represents the mean ± SD. (D) Representative images of MCF-7 xenograft tumors isolated from the control group, cisplatin (5 mg/kg/3d), VALD-3-treated groups and VALD-3 + cisplatin-treated group after treatment for 13 days. (E) Western blotting were performed to analyze the expression levels of Wnt/β-catenin pathway and its downstream molecules and (F) the expression levels of Surviving and XIAP in tumor tissues. (G) Survival analysis curve. (H) The apoptotic levels of human breast tumor tissues were measured by TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining. (I) Immunohistochemical analysis of Bad and BAX in tumor tissues. Pretreatment with VALD-3. (J) HE staining of tumor specimens and major organs. Scale bars = 100 µm.

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