Figure 2 | Scientific Reports

Figure 2

From: Identification of pathological transcription in autosomal dominant polycystic kidney disease epithelia

Figure 2

Transcriptomic profiling of genetically engineered ADPKD cells showed significant interdependence of Pkd1 and Pkd2 mRNA expression. (a) Unsupervised hierarchical clustering showed low variability and genotype-dependent grouping of mIMCD3 Wt, Pkd1−/− and Pkd2−/− cells. (b) Pkd1 knockout was generated by a full genomic deletion. Loss of Pkd2 was associated with a significant transcriptional up-regulation of Pkd1 mRNA (782.25 [SEM = 97] mean normalized counts in wildtype vs. 1,209 [SEM = 175] in Pkd2−/− cells). (c) Pkd2 knockout was based on genomic deletion of Pkd2 exons 4–9 and no counts were detected for these exons. + : adjusted p-values for single exons 4–9 were < 1.75 × 10–51 in Pkd2−/− versus Wt and Pkd2−/− versus Pkd1−/− cells.

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