Figure 6 | Scientific Reports

Figure 6

From: Identification of pathological transcription in autosomal dominant polycystic kidney disease epithelia

Figure 6

Cross-species meta-analysis of independent ADPKD transcriptomic profiles. (a) 7 independent Pkd1−/− transcriptomic studies were chosen for a meta-analysis approach, comprising cell culture, mouse tissue as well as human kidney samples12,13,14,15,16,18. 6 studies had been previously published, 3 in another meta-analysis15. 1 mouse model (Pkd1−/− RNA-seq) was established for independent confirmation (Figure S5). 31 genes were identified in the core data set and at least 3 other studies. 61 genes are reported in the core data set only. (b) Of 31 confirmed genes Rep31, 2 showed concordant upregulation, 4 downregulation. Interestingly, 10 genes are concordantly regulated in cell culture models with discordant regulation in animal/human models. (c) Complementing published data sets, CD178 combined regulation by Pkd1−/− and Pkd2−/−. A total of 61 genes appeared in CD178 only (New61). (d) Combining the cross-species meta-analysis with function-based mapping and gene enrichment analysis of ABC25 highlighted 8 yet unreported and 6 confirmed genes. Both, confirmed and new genes were represented in clusters A10 and B8. Interestingly, none of the Rep31 but 3 of New61 genes were represented in functional cluster C7. Thus, combining Pkd1−/− and Pkd2−/− identified new targets for in-depth analysis of ADPKD pathogenesis.

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