Figure 5 | Scientific Reports

Figure 5

From: Roles for B[a]P and FICZ in subchondral bone metabolism and experimental temporomandibular joint osteoarthritis via the AhR/Cyp1a1 signaling axis

Figure 5

Mandibular subchondral bone volume and architecture are restored in FICZ-treated TMJ-OA mice. (A) A jaw opening device was applied to interincisal teeth of mice to achieve a maximal open posture. (B) Protocols and Experimental design. Mechanical daily overloadings were equally applied to bilateral TMJs with forced mouth-opening performed for 3 h each day. Charcoal or black arrows represent mechanical stress and administration with vehicle or FICZ, respectively. TRAP staining, micro-CT, TUNEL staining, and immunostaining analyses were performed according to protocol. (C) Representative transverse and sagittal view sections from micro-CT 3D scans of mandibular condyle sections from control, vehicle, and FICZ-treated TMJ-OA mice (FICZ low: 100 μg/kg, FICZ high: 100 mg/kg) are respectively shown. Scale white bar = 500 μm. (D–F) Values for the ratio between BV/TV (D), Tb.Th (E), and then Tb.Sp (F) are presented. (n = 7 per each group). **p < 0.01; *p < 0.05.

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