Figure 5
From: A functional ultrasound brain GPS for automatic vascular-based neuronavigation
Registration accuracy estimation based on super-localization imaging. Successive and time-delayed registrations are used to position the probe and image the same coronal or sagittal slice. Shifts from reconstructed images from several trials can be estimated as 3D translations enabling the evaluation of the registration process. (A) Power Doppler images from a pair of acquisitions are overlaid both in coronal and sagittal directions and for both intra-animal and inter-animal data registrations. Misalignment cannot be correctly estimated with this level of detail (100 μm resolution). (B) Corresponding super-localization images, as microbubbles density reconstructed with 5 μm pixels. Scale bar is 500 μm. (C) Zooming boxes showing finer local misalignment. Displacement map was computed as 2D translations between a pair of images and averaged over the whole images and over both pairs of acquisitions to evaluate intra-animal and inter-animal data registration accuracy in the 3 space directions as Δx (lateral error from coronal acquisitions), Δy (elevation error from sagittal acquisitions) and Δz (axial error from both coronal and sagittal acquisitions). Scale bar is 200 μm within zoomed boxes.
