Figure 3 | Scientific Reports

Figure 3

From: Author Correction: Strictly regulated agonist-dependent activation of AMPA-R is the key characteristic of TAK-653 for robust synaptic responses and cognitive improvement

Figure 3

Effects of TAK-653 on BDNF expression in vitro and in vivo. (A) Effects of TAK-653 on BDNF protein levels in rat primary hippocampal neurons. Cells were treated with AMPA (0 or 1 μM) and TAK-653 (0.01, 0.1, 1 μM) for 24 h and then were collected using lysis buffer. Cells in control group were treated with 1 μM AMPA and DMSO. Values were expressed as pg per mL. Data are represented as mean ± SD (n = 3). Statistical significance was determined by a two-tailed Williams’ test with significance set at #P ≤ 0.05 (versus control group; two-tailed Williams’ test). (B) Effects of TAK-653 on BDNF mRNA in hippocampus in AMPA (3.5 mg/kg, i.v.)-treated mice. TAK-653 (3 and 10 mg/kg, p.o.) was administered to mice 1 h before the administration of AMPA (3.5 mg/kg, i.v.) (left) or vehicle (right). Tissues were isolated 3 h after AMPA administration. Data were presented as the mean ± SEM (n = 23–24). #P ≤ 0.05 (versus vehicle-treated group; two-tailed Williams’ test).

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