Figure 4
Effects of AAS + HTAB gels on mice skin. Gels were applied at the dorsal area of the trunk region of swiss albino mice for seven consecutive days and the effects recorded are presented through (A) photographs of treated skins and (B) corresponding histopathological evaluation of inflammation through micrographs. Systems: i, sterile distilled water (NC); ii, C12MalNa2 + HTAB gel (100 mM, 60/40, M/M) and iii, 5% (w/v) phenol-water (PC). Micrographic scale bar: 20 μm. (C) cumulative irritancy index (CII) scores for skins treated with different systems, calculated according to Organization for Economic Co-operation and Development (OECD) guidelines67. Overall comparison of CII scores among the groups were carried out by the Kruskal–Wallis test followed by post-hoc Dunn’s test for multiple comparisons between each pair of groups. Significant differences in CII scores at P < 0.05 were obtained between groups PC vs. NC, PC vs. C12MalNa2 + HTAB, PC vs. C12AspNa2 + HTAB, and PC vs. C12GluNa2 + HTAB gels.
