Table 2 Primers and annealing temperatures for qPCR assays.
Primer | Primer sequence (5′–3′) | Tm (℃) | Annealing time |
|---|---|---|---|
tet33-F | TCCCGCACTGCTACACGA | 55 | 15 s |
tet33-R | GCGAGATGGCACCGAACA | ||
cml_e8-F | CGTGCCAGCGAACCAGAAG | 55 | 15 s |
cml_e8-R | TGGGTGCCAGGAAGGTGAAT | ||
tetV-F | GCACTCGTCTTCTCCACCTT | 55 | 15 s |
tetV-R | GGTTCCCTCCAGCCCATTAG | ||
ant2ia-F | CATCCCGTGGCGTTATCC | 55 | 15 s |
ant2ia-R | CTGGGCAGGTAGGCGTTT | ||
sul1-F | CGCACCGGAAACATCGCTGCAC | 65 | 15 s |
sul1-R | TGAAGTTCCGCCGCAAGGCTCG | ||
16S rRNA-F | CCTACGGGAGGCAGCAG | 62 | 15 s |
16S rRNA-R | ATTACCGCGGCTGCTGG |
