Figure 2
From: Nucleotide-decorated AuNPs as probes for nucleotide-binding proteins
Two-step AuNP surface modification with 5’ cap analogs. (A) Citrate AuNPs are incubated with Tween80 overnight before adding the nucleotide solution (1a, 1b, or 1c) to stabilize the AuNPs during surface modification for further biding experiments with eIF4E. (B) Absorption spectra of AuNPs stabilized with citrate, Tween80 or 1a. (C) Normalized absorption spectra obtained by the titration of m7G-cap-AuNPs conjugates with eIF4E protein. (D) Differential absorption spectra obtained from the same titration experiment. (E) Absorption changes at 550 nm for m7G-cap-AuNPs as a function of increasing eIF4E concentration. (F) DLS hydrodynamic radii of AuNPs differing in surface composition. (G) DLS-determined radii of AuNPs modified with various cap analogs in the presence and absence of eIF4E (150 nM). The concentration of AuNPs in all experiments and at all modification stages was 600 pM, except for DLS measurements (150 pM).
