Figure 5
From: Nucleotide-decorated AuNPs as probes for nucleotide-binding proteins
Optimizing the 4E-BP1 conjugate for a two-component AuNP-based eIF4E sensor. (A) The design of the two component sensing system that enables the controlled aggregation of AuNP conjugates in the presence of eIF4E. (B) The eIF4E molecule in a m7GTP/4E-BP1 peptide complex (PDB ID:2V8W)44. Coding: eIF4E, green; 4E-BP1, red; nucleotide, balls and sticks. (C) Representative changes in the absorption spectra of the 4E-BP1-AuNP conjugate when titrated with eIF4E protein. (D) Titration of the same conjugate with unspecific protein (BSA). (E) Diluting the 4E-BP1 peptide on the surfaces of the gold nanoparticles results in a decrease in undesired aggregation in the presence of eIF4E. Absorption changes were measured at point of the largest absorption change (620 nm). (F) Aggregation rates upon addition of eIF4E protein for the solo 4E-BP1 conjugate and that mixed with m7G-cap-TL-AuNPs. The concentration of AuNPs in all experiments was 600 pM.
