Figure 2
RT-qPCR detection of candidate miRNA biomarkers in AKI patient urine samples and control subjects. (A-E) Significant differences were observed between AKI patients (n = 29) and controls (n = 10) for (A) miR-21 (sevenfold increase), (B) miR-126 (fourfold increase), (C) miR-141 (twofold increase), (D) miR-192 (50% decrease) and (E) miR-204 (50% decrease). (F) Receiver operating characteristic (ROC) curve comparing all AKI patients with controls for all five miRNAs gave an area under the curve (AUC) = 0.94. (G) Bar graphs of AUCs for single and multiple miRNA comparisons between AKI patients compared to controls. Statistical analysis comparing two groups was carried out by Mann–Whitney U test. Data were normalised to endogenous control miR-191 and are presented as median ± range; *p < 0.05, ***p < 0.001. CON: control; AKI ALL: all AKI patients; Top Three: miR-192, miR-21 and miR-204.
