Figure 4

Site-specific occupancy and overall extent of PTMs in beers from Newstead Brewing Co. (A) Total and (B) average glycosylation occupancy. (C) Heat map of normalised abundance of unmodified and glycosylated forms of tryptic peptide R-V₉₄ITGVPWYSTR₁₀₄-L from Pau15. Hex modification location was not determined. (D) Total and (E) average glycation occupancy. (F) Heat map of normalised abundance of unmodified and glycated forms of tryptic peptide K-M₃₆KPCLTYVQGGPGPSGECCNGVR₅₈-S from NLTP1. (G) Total and (H) average extent of proteolysis. (I) Heat map of normalised abundance of unmodified and proteolytically cleaved forms of full tryptic peptide R-V₅₆VDQQLVGQLPWSTGLQMQCCQQLR₈₀-D from GLT3. Column graphs were of either total of average PTM occupancy/extent, calculated as either sum or average of all modified peptides normalised to their peptide family, respectively. Heat maps show modified peptides normalised to their peptide family. Bars show mean, n = 3, Error bars show SEM, * indicates statistically significant (p < 0.05).