Figure 5
From: Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells
Correlation between ROS generation and intensity of cell-particle interaction. (A) Representative gating strategy for rising PCI. Gate NC represents cells with no particle interactions, I–V show increasing PCI (SSC = side scatter, FSC = forward scatter). (B) The respective ROS intensity for gated cells shown in (A). (C) Correlation between PCI, normalized ROS-quantity, and gated cell count. ROS quantity was normalized (control: cells incubated without particles). MFI is the mean SSC value of respectively gated cells. The dashed line highlights the relevant 100% ROS line, the back-ground ROS production determined in non-treated cells cultivated at otherwise similar conditions. Used particle concentration: hc Table 2 (black circle = 0.2 µm, orange circle = 0.5 µm, blue circle = 1 µm, green circle = 2 µm, yellow circle = 3 µm, purple circle = 6 µm).
