Figure 5

In vitro efficacy analysis of serum and spleen T cell isolated from challenge applied mice. (A) The bar graph showing the presence (absorbance) of SARS-CoV-2 specific IgG in the mice sera diluted with either 1:128 or 1:256 detected using ELISA. (B) The bar graph showing the neutralization frequency of the mice sera diluted to 1:128 or 1:256 that were pre-incubated with a 100 × TCID50 dose of infective SARS-CoV-2. The analysis was performed with MTT analysis at 96 h. (C) The bar graph showing the activation frequency (IFNγ positive spot count) of spleen T cells that were incubated with SARS-CoV-2 specific peptides. (D) The bar graph showing cytokine (IL-10, IL-2, IL-4, TNFa, and IL-12) proportions of spleen T cells that were incubated with SARS-CoV-2 specific peptides. (E) The bar graph showing a change in the frequency of the T cells after the stimulation. The activation of the T cells was determined with the upregulation of the CD25 surface marker.