Figure 1

Disruption of the Ypel4 gene results in a macrocytic secondary polycythemia. (A) Schematic of the Ypel4 wild-type (+) and knockout (−) allele in the mouse model (image adapted from https://www.mousephenotype.org⁴⁹). (B) Breeding statistics of breeding pairs carrying one wild-type and one knock-out allele (+/−) each (n = 19–45). (C) Peripheral blood analysis of hematocrit (HCT) (n = 13–19); (D) Hemoglobin concentration (HGB) (n = 13–19); (E) Red blood cell count (RBC) (n = 13–19); (F) Mean corpuscular volume (MCV) (n = 13–19); (G) Mean corpuscular hemoglobin (MCH) (n = 13–19); (H) Mean corpuscular hemoglobin concentration (MCHC) (n = 13–19) and (I) Red cell distribution width (RDW_SD) (n = 7) by utilization of a hematology analyzer. (J) Percentage of reticulocytes in total red blood cells as quantified by flow cytometry (n = 13). (K) Erythropoietin concentration in serum as detected by enzyme-linked immunosorbent assay (ELISA) (n = 9). Data displayed as average ± SEM, *P ≤ 0.05, ****P ≤ 0.0001.