Figure 1

Alpha-synuclein (α-syn) extracted from the pars intermedia of PPID-affected horses has the potential to cross seed recombinant human α-syn. (A) Thioflavin T (ThT) fluorescence assay kinetics of human α-syn fibril formation in the presence of equine α-syn isolated from young, aged, and PPID-affected horses. Data represent the mean of the fluorescence intensity with error bars for SEM obtained from three different horses in each group (young, aged, PPID). (B) Lag phase time (min) for initiation of human α-syn fibril formation during incubation with equine α-syn extracted from pars intermedia tissue collected from young, aged and PPID-affected horses. The time (min) required for human α-syn fibril elongation (lag phase) is shorter when induced with equine α-syn extracted from PPID-affected horses. Data represent the mean of the lag phase time (min) obtained from five different horses in each group (young, aged, PPID). Data were analyzed by the one-way analysis of variance with Dunnett's multiple comparison post-hoc testing between groups. (*significant difference p < 0.05.)