Figure 2

Alpha-synuclein (α-syn) extracted from the pars intermedia of PPID-affected horses exhibits a fibrillar ultrastructure as visualized by transmission electron microscopy (TEM). (A) TEM image of the background staining showing the absence of fibrils in equine α-syn isolated by immunoprecipitation (IP) from the pars intermedia of a young horse. (Scale bar, 100 nm.) (B) TEM image of α-syn isolated by IP from pars intermedia of an aged horse representing background staining (no fibrils). (Scale bar, 100 nm.) (C) TEM image of positively stained equine α-syn fibrils isolated by IP from the pars intermedia of a PPID-affected horse. (Scale bar, 100 nm.) (D) TEM image of a collagen fiber. (Scale bar, 200 nm.)