Table 2 Human (Homo sapiens) and horse (Equus caballus) α-synuclein (α-syn) amino acid sequences. The 140 amino acid α-syn protein consists of three regions: N-terminal amphipathic region (residues 1 to 60), hydrophobic non-amyloid-β component (NAC) (residues 61–95) and the acidic C-terminal region (residues 96–140). Tango bioinformatic aggregation scores (35–37) of the full-length human and equine α-syn are 896 and 1042, respectively. Aggregation scores for each segment of the human and equine α-syn.

Fragment

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

Variation

Agg score

Human

M

D

V

F

M

K

G

L

S

K

A

K

E

G

V

V

A

A

A

E

K

T

K

Q

G

57

Horse

M

D

V

F

M

K

G

L

S

K

A

K

E

G

V

V

A

A

A

E

K

T

K

Q

G

0

57

Fragment

26

27

28

29

30

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

46

47

48

49

50

Human

V

A

E

A

A

G

K

T

K

E

G

V

L

Y

V

G

S

K

T

K

E

G

V

V

H

291

Horse

V

A

E

A

A

G

K

T

K

E

G

V

L

Y

V

G

S

K

T

K

E

G

V

V

H

0

291

Fragment

51

52

53

54

55

56

57

58

59

60

61

62

63

64

65

66

67

68

69

70

71

72

73

74

75

Human

G

V

A

T

V

A

E

K

T

K

E

Q

V

T

N

V

G

G

A

V

V

T

G

V

T

117

Horse

G

V

T

T

V

A

E

K

T

K

E

Q

V

T

N

V

G

E

A

V

V

T

G

V

T

2

56

Fragment

76

77

78

79

80

81

82

83

84

85

86

87

88

89

90

91

92

93

94

95

96

97

98

99

100

Human

A

V

A

Q

K

T

V

E

G

A

G

S

I

A

A

A

T

G

F

V

K

K

D

Q

L

5

Horse

A

V

A

Q

K

T

V

E

G

A

E

S

I

A

A

A

T

G

F

G

K

K

D

H

L

3

8

Fragment

37

38

39

40

41

42

43

44

45

46

47

48

49

50

51

52

53

54

55

56

57

58

59

60

61

Human

V

L

Y

V

G

S

K

T

K

E

G

V

V

H

G

V

A

T

V

A

E

K

T

K

E

53

Horse

V

L

Y

V

G

S

K

T

K

E

G

V

V

H

G

V

T

T

V

A

E

K

T

K

E

1

39

Fragment

62

63

64

65

66

67

68

69

70

71

72

73

74

75

76

77

78

79

80

81

82

83

84

85

86

Human

Q

V

T

N

V

G

G

A

V

V

T

G

V

T

A

V

A

Q

K

T

V

E

G

A

G

483

Horse

Q

V

T

N

V

G

E

A

V

V

T

G

V

T

A

V

A

Q

K

T

V

E

G

A

E

2

637

Fragment

91

92

93

94

95

96

97

98

99

100

101

102

103

104

105

106

107

108

109

110

111

112

113

114

115

Human

A

T

G

F

V

K

K

D

Q

L

G

K

N

E

E

G

A

P

Q

E

G

I

L

E

D

0

Horse

A

T

G

F

G

K

K

D

H

L

G

K

S

E

E

G

A

A

Q

E

G

I

L

E

D

4

0

Fragment

116

117

118

119

120

121

122

123

124

125

126

127

128

129

130

131

132

133

134

135

136

137

138

139

140

Human

M

P

V

D

P

D

N

E

A

Y

E

M

P

S

E

E

G

Y

Q

D

Y

E

P

E

A

0

Horse

M

P

V

D

P

D

N

E

A

Y

E

M

P

S

E

E

G

Y

Q

D

Y

E

P

E

A

0

0