Table 2 Temporal expression of SfAV and TnAV genes coding for transmembrane domain containing proteins in tissues of their respective hosts, larvae of Spodoptera frugiperda and Trichoplusia ni, at 7, 14, and 21 days post-infection.

From: Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph

SfAV-1a genes with predicted transmembrane helix

Somatic

Hemolymph

7

14

21

7

14

21

ORF009 (DEAD-like helicase of the SNF2 family)

223.28

132.15

115.88

429.58

546.50

167.04

ORF035 (Lipid membrane protein)

620.83

447.44

378.36

1325.47

1792.00

600.44

ORF054 (Putative myristylated membrane protein)

132.27

95.43

118.61

157.20

252.66

139.14

ORF013 (Esterase/lipase)

67.31

45.96

33.57

31.04

47.27

50.03

ORF087 (Fatty acid elongase)

44.64

29.89

34.86

86.99

87.20

43.46

ORF093 (Patatin-like phospholipase)

20.96

23.48

32.81

51.90

74.58

43.24

ORF112 (Phosphate acyltransferase)

43.51

36.69

54.86

44.38

98.43

62.98

ORF110 (ATPase)

24.72

18.40

16.55

46.20

72.26

28.39

ORF086 (Uvr/REP helicase)

68.83

46.31

51.60

121.24

215.38

106.50

ORF014 (Zinc-dependent metalloproteinase)

30.95

26.64

18.79

44.65

103.21

41.87

ORF097 (RING finger domain)

1169.17

1221.30

1403.31

1959.94

2838.71

2073.95

ORF032 (Hypothetical protein)

1807.96

1300.95

1133.74

164.37

230.75

315.65

ORF062 (Hypothetical protein)

5.45

2.81

3.43

10.89

15.37

7.12

ORF108 (Hypothetical protein)

123.17

96.86

117.62

260.45

360.91

157.95

ORF119 (Hypothetical protein)

95.40

63.67

44.00

212.38

310.68

84.15

ORF007 (Hypothetical protein)

198.77

138.69

85.62

186.47

231.38

280.02

ORF012 (Hypothetical protein)

28.70

25.58

25.27

71.04

133.48

43.09

ORF039 (Hypothetical protein)

53.94

48.48

65.73

110.85

156.91

77.62

ORF045 (Hypothetical protein)

0.21

0.27

0.30

0.10

0.54

0.39

ORF056 (Hypothetical protein)

14.12

15.70

26.43

23.10

45.10

21.94

ORF085 (Hypothetical protein)

206.41

243.02

222.01

263.95

900.86

391.74

ORF092 (Hypothetical protein)

28.31

52.60

74.71

42.92

209.84

102.88

ORF122 (Hypothetical protein)

11.94

8.43

5.66

7.76

17.30

8.94

ORF002 (Hypothetical protein)

75.36

46.69

42.18

203.58

260.88

85.29

ORF043 (Hypothetical protein)

747.85

582.75

884.34

792.14

1388.86

675.05

ORF060 (Hypothetical protein)

745.33

577.04

1456.56

1033.36

1859.18

887.81

TnAV-6a1 genes with predicted transmembrane helixa

ORF102 (Cathepsin B)

1.29

1.31

0.52

73.41

38.56

13.16

ORF158 (Zinc metalloproteinase)

2.45

2.43

1.60

54.17

36.92

13.83

ORF098 (Phosphate acyltransferase)

3.81

3.46

1.15

219.29

98.14

30.06

ORF46_47 (Fatty acid elongase/elongation of very long chain fatty acids protein 1-like)b

37.89

32.49

7.27

1915.30

667.47

228.81

ORF067 (Patatin-like phospholipase)

40.55

33.51

10.50

2645.55

1031.08

290.99

ORF080 (Transcription repressor MOT2/ ubiquitin ligase)

19.80

12.05

5.53

523.56

235.73

78.46

ORF129 (Hypothetical protein/Myristylated membrane-like protein)

11.19

12.97

3.64

1080.61

368.79

100.86

ORF091 (Hypothetical protein)

2.97

2.65

1.61

77.50

32.83

10.12

ORF149 (Hypothetical protein)

340.68

313.75

114.74

20,771.67

7198.21

2683.23

ORF157 (Hypothetical protein)

2.13

2.86

0.96

154.05

80.24

44.71

ORF160 (Hypothetical protein)

1460.48

592.36

110.63

275.46

50.88

57.48

ORF023 (Hypothetical protein)

1666.32

545.18

357.25

2066.77

677.67

378.65

ORF060 (Hypothetical protein)

7.86

4.77

2.56

168.87

75.94

28.18

  1. The prediction of transmembrane helices in proteins was carried out by TMHMM Server v. 2.0. http://www.cbs.dtu.dk/services/TMHMM-2.0/.
  2. Data presented are the average of three replicates of the Reads Per Kilobase Per Million (RPKM) at each time point for somatic tissues versus hemolymph.
  3. The primary tissue initially infected by SfAV-1a in S. frugiperda is the fat body, whereas in T. ni the initial tissues infected by TnAV are the fat body, epidermis, and tracheal matrix.
  4. aThe TnAV-6a (TnAV-2c, previously)23 was used as a reference genome to refer to the ORFs order in our TnAV-6a1 variant.
  5. bThe _ sign refers to two ORFs that were found in our TnAV-6a1 variant strain as a single ORF.
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